1. Absolute quantification of translational regulation and burden using combined sequencing approaches
Comparison of protein synthesis rate of endogenous E. coli genes measured using Ribo‐seq from this study (in molecules/s units) and from that by Li et al () (in molecules/generation units). Each point corresponds to a single gene, and color denotes the ratio of transcription initiation rate to translation initiation rate (giving RNAP/ribosome) capturing whether transcription (light yellow) or translation (dark blue) is more dominant. Transcription (bottom) and translation (top) profiles for uspA, ompA, and gapA, computed from the RNA‐seq and Ribo‐seq data without induction. Positions of the genetic parts and gene are shown below the profiles. Promoter strengths in RNAP/s units and RBS initiation rates in ribosome/s units. Transcription (bottom) and translation (top) profiles for lacZ. Profiles are shown for cells in the absence and presence of IPTG (1 mM). Position of genetic parts and gene is shown below the profiles. RBS is omitted from the genetic design due to its size. Measured promoter strength in RNAP/s units, RBS initiation rate in ribosomes/s units, and the transcriptional terminator and translation termination efficiency for lacZ. Data shown for cells in the absence and presence of IPTG (1 mM).
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Mol Syst Biol, Volume: 15, Issue: 5, First published: 03 May 2019, DOI: ( /msb )