1. Expression of human tau in GFAP/tau Tg mice.
Expression of human tau in GFAP/tau Tg mice. A-C, Western blot analysis of HS-TBS-soluble proteins (10 μg per lane) extracted from cortex, brainstem (BS), and spinal cord (SC) samples of 2-month-old GFAP/tau Tg and control mice [non-Tg (NTg)]. Immunoblots were detected with antibodies that detect human tau only (T14; A), murine tau only (T49; B), or both human and murine tau (T46; C). D, Western blot analysis of native (-) and dephosphorylated (+) HS-TBS-soluble proteins extracted from brainstem and probed with T46 that recognizes both mouse and human tau. Arrows in C and D indicate migration of T34 human tau that is specifically expressed in GFAP/tau Tg mice. Molecular weight standards are indicated to the left of each panel. WT, Wild type; DeP, dephosphorylated.
Mark S. Forman et al. J. Neurosci. 2005;25:
©2005 by Society for Neuroscience