1. FOXO3a phosphorylation and expression.
FOXO3a phosphorylation and expression. A. Western blot analysis for phosphorylation of FOXO3a at Thr32 (anti-phospho-Thr32, UBI 1:250) in LNCaP and LNAI cells. Blots were reprobed for β-actin to control for loading and transfer variations. Signal intensities were normalized to that of β-actin and then divided by the FOXO3a total protein/β-actin ratio from a parallel blot to yield the normalized FOXO3aphosphoT32 values depicted graphically and numerically (inset). Columns, mean of LNCaP and LNAI lysates shown in the inset Western blots; bars, SE. B. Western blot analysis of FOXO3a protein expression in androgen-dependent LNCaP and the androgen-independent derivatives, LNAI and LNAI-2, using anti-FOXO3a/FKHRL1 antibody (UBI 1:250). The blot was reprobed for β-actin (1:10,000, Sigma, St. Louis, MO) to control for loading and transfer. Blots (A and B) are representative of more than five independent Western blot experiments.
Rebecca L. Lynch et al. Mol Cancer Res 2005;3:
©2005 by American Association for Cancer Research