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Ability of deoxyribonucleic acid–damaged sperm to withstand freeze-thaw–induced damage during cryopreservation  Satish Kumar Adiga, Ph.D., Zaheer Khan,

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Presentation on theme: "Ability of deoxyribonucleic acid–damaged sperm to withstand freeze-thaw–induced damage during cryopreservation  Satish Kumar Adiga, Ph.D., Zaheer Khan,"— Presentation transcript:

1 Ability of deoxyribonucleic acid–damaged sperm to withstand freeze-thaw–induced damage during cryopreservation  Satish Kumar Adiga, Ph.D., Zaheer Khan, B.Sc., Dinesh Upadhya, M.Sc., Guruprasad Kalthur, Ph.D., Pratap Kumar, M.D.  Fertility and Sterility  Volume 92, Issue 3, Pages (September 2009) DOI: /j.fertnstert Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 The incidence of sperm DNA damage in mouse testicular sperm after exposure to varying doses of γ-radiation. The number of TUNEL-positive sperm in the 5- and 10-Gy groups was significantly higher than in the 0-Gy (P<.001) and 2.5-Gy (P<.01) groups. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Sperm viability before (red bars) and after (green bars) cryopreservation in mouse testicular sperm exposed to various doses of γ-radiation. The difference in sperm viability between the respective groups was statistically significantly different (P<.01). ∗P<.05 vs. 0-, 2.5-, and 5-Gy respective groups. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 Regression analysis showing a strong negative correlation (R = −0.87) between the sperm DNA damage and postthaw survival. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

5 Figure 4 The incidence of sperm DNA damage before (red bars) and after (green bars) cryopreservation. The values between respective groups were statistically nonsignificant. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions


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