1. c-MYC controls expression of ABC drug transporters in CD34+ hematopoietic progenitors.
c-MYC controls expression of ABC drug transporters in CD34+ hematopoietic progenitors. A, relative expression of c-MYC and ABC genes in the CD34+ cell population was compared with that of the entire population of mononuclear cells (MNC). Each sample was analyzed in duplicate and threshold cycle (Ct) values were averaged. Results are reported as delta Ct values which were calculated as the difference between Ct of the ABC mRNAs and Ct of the GUSB mRNA used for normalization. B, c-MYC and ABC gene expression of CML CD34+ cells was compared with that of CD34+ cells from healthy donors. The 2 sets of data were compared by using the ΔΔCt method, with the GUSB (housekeeping gene) as internal reference and the medians of each gene expression values as calibrators. Both in (A) and (B) significant differences between the 2 cell populations were determined by using Mann–Whitney statistical tests.
Antonio Porro et al. Mol Cancer Res 2011;9:
©2011 by American Association for Cancer Research