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Analysis of PHO1 Expression by RT-qPCR in the pru1 Mutant, Complementation Lines (COM5 and COM7), and Wild-type Plants during Pi Starvation. Analysis of PHO1 Expression by RT-qPCR in the pru1 Mutant, Complementation Lines (COM5 and COM7), and Wild-type Plants during Pi Starvation. Seven-day-old seedlings were transferred to LP medium and the roots were harvested at the indicated time points for RNA extraction. Data are mean values of three biological replicates ± se. Asterisks indicate statistically significant differences compared with wild-type plants by Student’s t test: **P < 0.01. Qing Ye et al. Plant Cell 2018;30: ©2018 by American Society of Plant Biologists
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