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Eau d’coli N Kuldell for Spring 2011
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System level description: initial planning
Black box functions Input e.g. precursor Output e.g. smell WGD: Wintergreen Generating Device WGD BGD and BGD: Banana Generating Device
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System level description: scent production
WGD Black box functions Input e.g. precursor Output e.g. smell BGD WGD: Wintergreen Generating Device BGD: Banana Generating Device
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System level description: scent production
WGD Black box functions Input e.g. precursor Output e.g. smell BGD WGD: Wintergreen Generating Device BGD: Banana Generating Device
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System level description: precursor production
WGD Black box functions Input e.g. cell growth Output e.g. smell BGD saGD and iaGD WGD: Wintergreen Generating Device BGD: Banana Generating Device saGD: salicylic acid Gen Dev iaGD: isoamyl alcohol Gen Dev
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System level description: precursor production
Input e.g. cell growth saGD WGD Black box functions Output e.g. smell iaGD BGD WGD: Wintergreen Generating Device BGD: Banana Generating Device saGD: salicylic acid Gen Dev iaGD: isoamyl alcohol Gen Dev
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System level description: growth regulation
Input e.g. cell growth saGD WGD Output e.g. smell iaGD BGD cell density time “stationary” “log” “lag”
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System level description: growth regulation
Input e.g. log growth saGD WGD ON OFF iaGD BGD cell density time “lag” “log” “stationary” wintergreen smell
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System level description: growth regulation
Input e.g. stationary growth saGD WGD OFF ON iaGD BGD cell density time “lag” “log” “stationary” banana smell
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System level description: growth regulation
Input e.g. stationary growth saGD WGD OFF ON iaGD BGD OsmY is a promoter that is only active during stationary phase
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System level description: growth regulation
Input e.g. stationary growth saGD WGD OFF ON iaGD BGD Stationary phase Log phase OsmY OsmY+ inverter
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System level description: growth regulation
Input e.g. stationary growth saGD WGD OFF ON iaGD BGD
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System level description: Chassis modification
Input e.g. stationary growth saGD WGD OFF ON iaGD BGD eliminate natural indole production
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System level test: gas chromatographs
methyl salicylate MW = 152 no methyl salicylate [no peak at 9.1 minutes] E. coli E. coli + precursor (SA) + WGD
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System level test: uninformed experimental subjects??
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