1. Effect of the PD-1–B7-H1 interaction on cell proliferation potential and drug sensitivity in MOSTI-1 cells.
Effect of the PD-1–B7-H1 interaction on cell proliferation potential and drug sensitivity in MOSTI-1 cells. Cell-cycle analysis (A) and BrdUrd incorporation (B) in PD-1-Fc bead–treated MOSTI-1 cells were analyzed using FCM after 5 × 105 MOSTI-1 cells mixed with 2.5 × 106 PD-1-Fc or CD80-Fc beads were seeded in 48-well plates and cultured for 2 days. Sensitivity to melphalan (C) or bortezomib (D) in PD-1-Fc or CD80-Fc bead–treated MOSTI-1 cells. Inhibition of melphalan resistance of PD-1-Fc bead–treated MOSTI-1 cells by anti–B7-H1 (E). The cells were preincubated with 10 μg/mL of monoclonal antibody to B7-H1 or control Ig at 4°C for 30 minutes and then treated with the beads for 24 hours. After incubation with melphalan for another 24 hours, cells with melphalan-induced apoptosis were detected using FCM. Melphalan induced apoptosis in PD-1-Fc bead–treated mock.KMS28-PE and B7-H1.KMS28-PE cells (F). The data are expressed as mean ± SD of triplicate experiments. *, P < 0.05; **, P < 0.01.
Mariko Ishibashi et al. Cancer Immunol Res 2016;4:
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