1. T-VISA is robust in ovarian cancer cell lines.
T-VISA is robust in ovarian cancer cell lines. A, schematic diagram of engineered T-VISA constructs in the pGL3 backbone. B, using a panel of ovarian cancer cell lines, normal ovarian epithelial cells (NOE99, NOE115, and NOE119), and normal lung fibroblasts (WI-38), plasmid DNA and pRL-TK were transiently cotransfected with the indicated. Forty-eight hours later, dual luciferase ratio was measured and shown as relative light units (ratio) normalized to the Renilla luciferase control. The data are means of four independent experiments. Bar, SD. C, the percentage of T promoter activity normalized to that of the CMV promoter.
Xiaoming Xie et al. Mol Cancer Ther 2009;8:
©2009 by American Association for Cancer Research