1. What the zebrafish core facility can offer you:
The zebrafish core facility at Karolinska Institutet
Bringing your basic and translational research to the next level
What the zebrafish core facility can offer you:
Embryos and adult fish of various wildtype and transgenic lines
Husbandry and breeding of your own zebrafish lines
Three complete work stations for microinjections
Work stations for bright-field and epifluorescence microscopy
Training in various zebrafish specific techniques like microinjection, transplantation, in situ hybridization, drug exposures etc.
Support in project planning
Practical assistance with your experiments
Equipment of the zebrafish core facility
Housing for ~ adult zebrafish
Large nursery for embryonic zebrafish
Large quarantine laboratory
2 wet laboratories
1 dry laboratory
3 work stations for microinjection
2 work stations for epifluorescence microscopy
2 microcapillary puller
The zebrafish – a brief introduction
Small, tropical freshwater fish originating from the Himalayan region
Embryos transparent, allowing for in vivo/real time analysis
Easy genomic manipulation
Extremely fast development (beating heart after 24 hours, freely swimming after 72 hours!)
Powerful model for developmental biology
Clinically relevant model for translational research and drug discovery
Only vertebrate model suitable for high-throughput screens
The zebrafish core facility – an overview
With a capacity of housing ~ adult zebrafish in a sophisticated and highly-controlled environment, our zebrafish core facility is the largest facility in Sweden. In 2016, we have provided amongst others more than embryos to 25 research groups at Karolinska Institutet. We have currently 4 animal technicians that can assist you with your project.
Why zebrafish?
Proven similarity of developmental circuits and processes to mammalian systems (e.g. hematopoiesis, angiogenesis, organogenesis etc.)
The transparent zebrafish embryos develop ex-utero, offering unique in vivo/real time imaging possibilities
Many transgenic lines facilitate imaging
The fully sequenced genome is easily accessible, fast transient knock-downs are rapidly possible
The past decade has proven its clinical relevance for various major health issues including cancer and neurodegeneration
Unique model to study processes in physiological hypoxia (e.g. tumor dissemination, drug profiling)
The only vertebrate model suitable for high-throughput drug discovery
The core facility management has more than 10 years experience of working with the model organism zebrafish and a large international network in the zebrafish community. We will do everything we can to support your project.
Where to find us:
Department of Medical Biochemistry and Biophysics
Entrance floor – Building B
Scheelesväg 2
17177 Stockholm
How to contact us:
core facility:
core facility manager: