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Use of sex chromosome bivalent pairing in spermatocytes of nonobstructive azoospermic men for the prediction of successful sperm retrieval Leah Yogev, Ph.D., Einav Zeharia, M.Sc., Sandra E. Kleiman, Ph.D., Batia B. Maymon, Ph.D., Ron Hauser, M.D., Amnon Botchan, M.D., Haim Yavetz, M.D., Gedalia Paz, Ph.D. Fertility and Sterility Volume 86, Issue 1, Pages (July 2006) DOI: /j.fertnstert Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions
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FIGURE 1 Percentage of spermatocytes with XY chromosome bivalent in the three groups: I) spermatocyte arrest; II) mixed atrophy; and III) obstructive azoospermia. XY bivalents were determined by the short arm (p) telomeres pairing (A), long arm (q) telomeres in proximity (B), all four telomeres in a cluster (C), and the proximity of centromeres (D), plotted with “box and whiskers.” The box encloses the middle half of the data (supplying first and third quartile), and is bisected by the median value. The vertical lines at the top and bottom of the box indicate the range of “typical” data values. Extreme values are displayed as an open circle for possible outlier (values that are outside the box boundaries by ≥1.5 times the size of the box) and asterisks for probable outliers (values that are outside the box boundaries by >3 times the size of the box). Yogev. Sex body and spermatogenesis completion. Fertil Steril 2006. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions
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FIGURE 2 Scatter plots with regression lines showing association between rate of spermatocytes with XY bivalent (first study, all three groups; n = 23) detected by X and Y chromosome centromeres in proximity and pairing of p telomeres (r = 0.85), q telomeres in proximity (r = 0.89), and four telomeres in cluster (r = 0.93). Yogev. Sex body and spermatogenesis completion. Fertil Steril 2006. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions
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