1. a, ATP production by PC3 prostate cancer cells after treatment with oligonucleotide/Lipofectin complexes.
a, ATP production by PC3 prostate cancer cells after treatment with oligonucleotide/Lipofectin complexes. Cells were treated and harvested as described in “Materials and Methods.” Cellular ATP samples were normalized to protein content as determined by the Bradford assay. Experiments were done in triplicate. Columns, mean; bars, SD. *, P < 0.05, by Student's t test assuming unequal variances. (Control not included in analysis: All comparisons made to G4126 to eliminate nonspecific oligonucleotide treatment.) b, flow cytometric analysis of the mitochondrial membrane potential of PC3 prostate cancer cells as assessed by JC-1 staining. %R1 are cells with depolarized mitochondria, as measured by the reversible shift in JC-1 fluorescence emission from red (FL-2 channel) to green (FL-1 channel). A positive control was performed using 1-mm H2O2 treatment for 1 h. The results from both the ATP production study and the evaluation of mitochondrial membrane potential indicate the integrity of mitochondrial respiration after treatment with oligomer/Lipofectin complexes.
Jonathan C. Lai et al. Mol Cancer Ther 2003;2:
©2003 by American Association for Cancer Research