1. Changes in levels of transcripts encoding inflammatory cytokines and GFAP. The amounts of the PCR cDNA products were obtained from phosphorimager readings of the bands in each blot and were expressed as a ratio to that of their respective control specimens ...
Changes in levels of transcripts encoding inflammatory cytokines and GFAP. The amounts of the PCR cDNA products were obtained from phosphorimager readings of the bands in each blot and were expressed as a ratio to that of their respective control specimens in the same blot. Each value in the graph represents the mean phosphorimager readings from two animals within the same blot; results from other blots using two additional animals from each group resulted in similar findings. Note that the elevation of IL-1α precedes that of GFAP, whereas those for TNF-α and IL-1β occur at around the same period as that for GFAP transcript. No increase in IFN-γ transcript is detected, and actin levels are relatively stable across the different groups to indicate relative similarity in the total RNA content in all groups.
Maria Rostworowski et al. J. Neurosci. 1997;17:
©1997 by Society for Neuroscience