1. Fig. 5 Simultaneous absence of caspase-3 and -7 is required for significant decrease of caspase-8 and -9 activation in intrinsic apoptosis.
Simultaneous absence of caspase-3 and -7 is required for significant decrease of caspase-8 and -9 activation in intrinsic apoptosis. (A) NALM6 WT or caspase-3/7−/− cells were left untreated or treated for the indicated time with ABT263 5 μM. Cells were lysed and processed for Western blot. (B to E) NALM6 WT or caspase-3/7−/− cells were left untreated or treated 22 hours with ABT263 at a concentration of 5 μM. WT NALM6 cells treated with ABT263 show metabolic activity for caspase-8 (B and C) and caspase-9 (D and E) substrates. Caspase-3/7−/− cells show no increased metabolic activity for caspase-8 (B and C) and caspase-9 (D and E) compared to untreated cells. Quantitation of caspase-8 and -9 activity, respectively. Histograms show representative data from at least two independent experiments performed in duplicate, with the mean from these ± SEM presented in the line graphs.
Scott McComb et al. Sci Adv 2019;5:eaau9433
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