1. Potentiation of proton-gated current in CHO cells expressing ASICs
Potentiation of proton-gated current in CHO cells expressing ASICs. Whole-cell patch clamp, holding potential −60 mV.
Potentiation of proton-gated current in CHO cells expressing ASICs. Whole-cell patch clamp, holding potential −60 mV. A, ASIC1a: application of pH 6.9 solution (open bar) before (left trace), during (middle trace), and after (right trace) the application of 100 μm SNAP (black bar). B, Corresponding bar graph in which the current is normalized to the control (100%, left column; n = 9; **p < 0.01). C, Current in ASIC1a transfected CHO cells in response to low pH in the absence (top) and in the presence (black box) of 100 μm SNAP. D, Dependence of peak current on pH in absence (open squares) and presence (black squares) of SNAP (n = 7). E–G, Enhancement of activity of ASIC1b, ASIC2a, and ASIC3 by SNAP (5 s pulses of pH 6.0, 6.0, and 6.9 respectively). *p < 0.05; **p < 0.01.
Hervé Cadiou et al. J. Neurosci. 2007;27:
©2007 by Society for Neuroscience