Presentation is loading. Please wait.

Presentation is loading. Please wait.

Intracellular TNF-α and IL-4 expression in gated CD33+ monocytes by three-color flow cytometry. Intracellular TNF-α and IL-4 expression in gated CD33+

Published byЕгор Озеров Modified over 5 years ago

Similar presentations

Presentation on theme: "Intracellular TNF-α and IL-4 expression in gated CD33+ monocytes by three-color flow cytometry. Intracellular TNF-α and IL-4 expression in gated CD33+"— Presentation transcript:

1 Intracellular TNF-α and IL-4 expression in gated CD33+ monocytes by three-color flow cytometry.
Intracellular TNF-α and IL-4 expression in gated CD33+ monocytes by three-color flow cytometry. PBMCs cultured for 6 h in medium (left set of panels) or stimulated with PMA-ionomycin (middle set of panels) or PHA (right set of panels) in the presence of monensin were labeled for surface expression of CD33 (PE/Cy5) and CD14 (FITC) and for intracellular TNF-α or IL-4 by using PE-conjugated MAbs. CD33+ gated monocytes were analyzed on FL1 (FITC) versus FL2 (PE) dot plots to discriminate cytokine expression in CD14− and CD14+ monocytes. Data from one representative experiment of five experiments performed are shown. Jarołsaw Baran et al. Clin. Diagn. Lab. Immunol. 2001; doi: /CDLI

Download ppt "Intracellular TNF-α and IL-4 expression in gated CD33+ monocytes by three-color flow cytometry. Intracellular TNF-α and IL-4 expression in gated CD33+"

Similar presentations

A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.

A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.
Laser Flow Cytometry Forward Scatter indicates size Forward Scatter.

Laser Flow Cytometry Forward Scatter indicates size Forward Scatter.
Retrovirally Transduced CD34++ Human Cord Blood Cells Generate T Cells Expressing High Levels of the Retroviral Encoded Green Fluorescent Protein Marker.

Retrovirally Transduced CD34++ Human Cord Blood Cells Generate T Cells Expressing High Levels of the Retroviral Encoded Green Fluorescent Protein Marker.
Fig. 1. TLR4 expression is much greater on macrophages than on DCs

Fig. 1. TLR4 expression is much greater on macrophages than on DCs
Volume 125, Issue 2, Pages (August 2003)

Volume 125, Issue 2, Pages (August 2003)
Fig. 2. Comparison of the Th1/Th2 ratio and the percentages of Th1 and Th2 cells between normal and allergic individuals. (A) The Th1/Th2 ratio observed.

Fig. 2. Comparison of the Th1/Th2 ratio and the percentages of Th1 and Th2 cells between normal and allergic individuals. (A) The Th1/Th2 ratio observed.
Clinical “cytokine storm” as revealed by monocyte intracellular flow cytometry: correlation of tumor necrosis factor α with severe gut graft-versus-host.

Clinical “cytokine storm” as revealed by monocyte intracellular flow cytometry: correlation of tumor necrosis factor α with severe gut graft-versus-host.
Representative flow cytometry dot plots from a healthy individual

Representative flow cytometry dot plots from a healthy individual
Fig. 1. IL-21 induces proliferation in CB-derived CD56+ populations. CB/CD34+ (A), and CB/CD56+ (B) cells were cultured.

Fig. 1. IL-21 induces proliferation in CB-derived CD56+ populations. CB/CD34+ (A), and CB/CD56+ (B) cells were cultured.
Melanoma donor (MD) NK cells are functionally impaired/exhausted.

Melanoma donor (MD) NK cells are functionally impaired/exhausted.
Human Immunodeficiency Virus Type 1 Protease Inhibitor Modulates Activation of Peripheral Blood CD4+ T Cells and Decreases Their Susceptibility to Apoptosis.

Human Immunodeficiency Virus Type 1 Protease Inhibitor Modulates Activation of Peripheral Blood CD4+ T Cells and Decreases Their Susceptibility to Apoptosis.
Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response  Miriam Wittmann, MD, Mareike Alter, Tanja.

Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response  Miriam Wittmann, MD, Mareike Alter, Tanja.
Further Analysis of Interleukin-2 Receptor Subunit Expression on the Different Human Peripheral Blood Mononuclear Cell Subsets by Denis David, Lynda Bani,

Further Analysis of Interleukin-2 Receptor Subunit Expression on the Different Human Peripheral Blood Mononuclear Cell Subsets by Denis David, Lynda Bani,
CD44-Mediated Adhesiveness of Human Hematopoietic Progenitors to Hyaluronan Is Modulated by Cytokines by Stéphane Legras, Jean-Pierre Lévesque, Rachida.

CD44-Mediated Adhesiveness of Human Hematopoietic Progenitors to Hyaluronan Is Modulated by Cytokines by Stéphane Legras, Jean-Pierre Lévesque, Rachida.
Galectin-9 binding to Tim-3 renders activated human CD4+ T cells less susceptible to HIV-1 infection by Shokrollah Elahi, Toshiro Niki, Mitsuomi Hirashima,

Galectin-9 binding to Tim-3 renders activated human CD4+ T cells less susceptible to HIV-1 infection by Shokrollah Elahi, Toshiro Niki, Mitsuomi Hirashima,
Human dendritic cells express neuronal Eph receptor tyrosine kinases: role of EphA2 in regulating adhesion to fibronectin by Blandine de Saint-Vis, Caroline.

Human dendritic cells express neuronal Eph receptor tyrosine kinases: role of EphA2 in regulating adhesion to fibronectin by Blandine de Saint-Vis, Caroline.
Expansion of FOXP3high regulatory T cells by human dendritic cells (DCs) in vitro and after injection of cytokine-matured DCs in myeloma patients by Devi.

Expansion of FOXP3high regulatory T cells by human dendritic cells (DCs) in vitro and after injection of cytokine-matured DCs in myeloma patients by Devi.
FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization  Sophia Björkander, Maria.

FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization  Sophia Björkander, Maria.
The heat shock protein Gp96 binds to human neutrophils and monocytes and stimulates effector functions by Markus P. Radsak, Norbert Hilf, Harpreet Singh-Jasuja,

The heat shock protein Gp96 binds to human neutrophils and monocytes and stimulates effector functions by Markus P. Radsak, Norbert Hilf, Harpreet Singh-Jasuja,
Interaction between B7-H1 and PD-1 determines initiation and reversal of T-cell anergy by Fumihiko Tsushima, Sheng Yao, Tahiro Shin, Andrew Flies, Sarah.

Interaction between B7-H1 and PD-1 determines initiation and reversal of T-cell anergy by Fumihiko Tsushima, Sheng Yao, Tahiro Shin, Andrew Flies, Sarah.

Similar presentations

Ads by Google