1. Induction of CDCP1 is regulated by Ras in NSCLC cells.
Induction of CDCP1 is regulated by Ras in NSCLC cells. A, analysis of CDCP1 mRNA expression by qRT-PCR in H322 cells transfected with wild-type (H-Ras-WT) or active Ras (H-Ras-12V) plasmid. B, H322 cells transfected with each plasmid were analyzed by immunoblotting. Each cells was cultured by serum-depleted medium. C, A549 cells were treated with K-Ras siRNA for 72 hours and detected expression of CDCP1. D, A549 cells treated with PD98059 or DMSO for 24 hours were analyzed by immunoblotting. E, analysis of CDCP1 mRNA expression by qRT-PCR in A549 cells treated with PD98059 or DMSO for 24 hours. Each data are the means ± SD from three experiments. *, P values in a Student t test < Black arrowheads denote band corresponding to CDCP1. White arrowhead indicates nonspecific bands.
Takamasa Uekita et al. Mol Cancer Res 2014;12:
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