1. CD49b+ cells from tumor-bearing mice are more prone to conversion into MDSCs compared with naive CD49b+ cells.
CD49b+ cells from tumor-bearing mice are more prone to conversion into MDSCs compared with naive CD49b+ cells. A, CD49b+ cells isolated from naive mice and 3- and 5-week tumor-bearing mice were incubated with 20 ng/mL of the indicated cytokines. On day 5, Ly6C/G and CD49b expression were analyzed by FACS. The numbers in the plot indicate the percentage of gated cells. B, the expression of cytokine receptor genes in freshly isolated CD49b+ cells from naive mice and 3- and 5-week tumor-bearing mice was measured using quantitative real-time PCR. The specific gene expression was normalized to the Hprt gene. The data represent the mean ± SEM. C, the kinetics of transcription factor expression during stimulation with GM-CSF or IL-2/GM-CSF. The data are representative of 3-independent experiments. **, P < 0.01; ***, P <
Young-Jun Park et al. Cancer Res 2013;73:
©2013 by American Association for Cancer Research