1. ARC Hub Knowledge & Technology
What’s ready to use?
2015

2. Genetic Relationship Matrix
Informed decision on genetic foundations when establishing a breeding population
Jarrod Guppy

3. Viral Presence and Prevalence
GAV low to high
YHV7 low to moderate
GAV undet. to very low
YHV7 undet.
GAV undet. to low
YHV7 undet. to very low
GAV moderate to high
YHV7 undet. to very low
GAV undet. to very low
YHV7 undet.
GAV is present amongst all wild stocks where broodstock are sourced
YHV7 is yet to be detected in the Cape van Diemen, Melville/Tiwi Island area
Jeff Cowley

4. Water transmission of GAV is difficult.
Mode of Transmission
Juvenile Black Tiger prawns submersed in water containing GAV inoculum had 100% survival and significantly less GAV compared to controls
Water transmission of GAV is difficult.
Juvenile Black Tiger prawns fed GAV infected prawn meat had ~50% survival and significantly more GAV compared to controls but loading was highly variable and uncontrolled.
GAV challenge assays are most reproducible and reliable when inoculum is injected into tail-muscle.
Tansyn Noble

5. Moderate heritability Low heritability
Genomic Prediction
Moderate heritability
Low heritability
Inbreeding
Response
5 families
100 families
5 families
100 families
To keep inbreeding at acceptable levels after 10 generations a breeding program needs to maintain 100 families
Mehar Khatkar

6. Industrial Scale Phenotyping
PhenoBox, a high-throughput digital capture phenotyping pipeline measuring weight, colour and body proportions of 24 prawns simultaneously and instantaneously
Near Field Infrared Spectroscopy (NIRS) to rapidly collect phenotypic data on protein, lipid and moult stage of prawns
Tissue sampling for nucleic acid extraction (genotyping)
On-farm phenotyping pipeline to process 2,000 prawns per day now exists
Nick Wade

7. On-farm stress testing for family rankings
Resilient Families
Susceptible Families
An on-farm 2 hour combined salinity and ammonia stress test for PL15 developed
Andrew Foote
Erika Giardina

8. Find out more and follow our progress at
2015