Presentation is loading. Please wait.

Presentation is loading. Please wait.

Registry of Parts: http://parts.mit.edu.

Published byBo Berg Modified over 5 years ago

Similar presentations

Presentation on theme: "Registry of Parts: http://parts.mit.edu."— Presentation transcript:

1 Registry of Parts:

2 Types of Parts:

3 Registry of Parts:

4 Registry of Parts:

5 BioBrick Parts Assembly Strategy
XbaI Spe1 EcoR1 PstI XbaI SpeI Mixed Site A CTAGA TGATC T

6 BioBricks Strategy: To add Downstream Parts
BioBrick Vector BioBrick Part EcoR1 XbaI PartA Spe1 PstI EcoR1 XbaI PartB Spe1 PstI To add to end Cut with Spe1 and Pst1 To add to end Cut with Xba1 and Pst1 EcoR1 XbaI PartA Spe1 PstI XbaI PartB Spe1 PstI Ligate (Xba1 and Spe1 Have compatible Overhangs) EcoR1 XbaI PartA Mixed PartB Spe1 PstI Site A CTAGA TGATC T

7 BioBricks Strategy: To add Upstream Parts
BioBrick Vector BioBrick Part EcoR1 XbaI PartA Spe1 PstI EcoR1 XbaI PartB Spe1 PstI Cut with EcoR1 and XbaI Cut with EcoR1 and Spe1 EcoR1 XbaI PartA Spe1 PstI EcoR1 XbaI PartB Spe1 Ligate (Xba1 and Spe1 Have compatible Overhangs) EcoR1 Xba1 PartB Mixed PartA Spe1 PstI Site A CTAGA TGATC T

8

9 Modified BioBrick-Like Strategy
AvrII Nhe1 Spe1/Xba1 Mixed Site ACTAGA TGATCT EcoR1 Xba1 Spe1 Pst1 Thr Arg Modified BioBrick EcoR Spe1 Nhe1 Pst1 GCT AGT Ala Ser

Download ppt "Registry of Parts: http://parts.mit.edu."

Similar presentations

Supplementary Figure 1A

Supplementary Figure 1A
Recombinant DNA prepare foreign (target) DNA prepare vector (host)

Recombinant DNA prepare foreign (target) DNA prepare vector (host)
Valerie Wisco & Casey Durnan. General Background Organism: Geobacter sulferreducens have the ability to transfer electrons on to the surface of electrodes.

Valerie Wisco & Casey Durnan. General Background Organism: Geobacter sulferreducens have the ability to transfer electrons on to the surface of electrodes.
Design and Testing of a Bacteriological Timer Device Herman Armstrong Morgan Schiermeier Rachel Klapper Jackie Schneider.

Design and Testing of a Bacteriological Timer Device Herman Armstrong Morgan Schiermeier Rachel Klapper Jackie Schneider.
Human Factor X Jessica Kettleson, Jordan Smith, and Tracy Cronbaugh.

Human Factor X Jessica Kettleson, Jordan Smith, and Tracy Cronbaugh.
DNA Manipulation in Synthetic Biology. DNA structure Covalent bondsHydrogen bonds.

DNA Manipulation in Synthetic Biology. DNA structure Covalent bondsHydrogen bonds.
Protein domain BioBricks Perry Tsai June 19, 2006.

Protein domain BioBricks Perry Tsai June 19, 2006.
UNIT 2 MANIPULATION OF DNA AND GENE ISOLATION LECTURES: 9. DNA Cloning and Library Construction 10. Isolating Genes.

UNIT 2 MANIPULATION OF DNA AND GENE ISOLATION LECTURES: 9. DNA Cloning and Library Construction 10. Isolating Genes.
ECOLITASTER: Cellular Biosensor Valencia iGEM 2006.

ECOLITASTER: Cellular Biosensor Valencia iGEM 2006.
DNA aSsEmBlY tEcHnIqUes Design and construct novel biological organisms programmed by genetic circuits using standardized biological parts called BioBricks.

DNA aSsEmBlY tEcHnIqUes Design and construct novel biological organisms programmed by genetic circuits using standardized biological parts called BioBricks.
11 Biobrick Jiale Wang SUSTC. 2 Definition What is Biobrick ? BioBrick standard biological parts are DNA sequences of defined structure and function;

11 Biobrick Jiale Wang SUSTC. 2 Definition What is Biobrick ? BioBrick standard biological parts are DNA sequences of defined structure and function;
Cloning Vector Map.

Cloning Vector Map.
Restriction Mapping.

Restriction Mapping.
Mutations of the alternate start signal for the Galls protein in Agrobacterium rhizogenes Henry McNett Dr. Walt Ream Department of Microbiology.

Mutations of the alternate start signal for the Galls protein in Agrobacterium rhizogenes Henry McNett Dr. Walt Ream Department of Microbiology.
Restriction Endonucleases BIO450. Restriction Enzymes Enzymatic Activity Biological Role Diversity Recognition Sequence Digestion Conditions Typical Reaction.

Restriction Endonucleases BIO450. Restriction Enzymes Enzymatic Activity Biological Role Diversity Recognition Sequence Digestion Conditions Typical Reaction.
PCR Product Orientation Test T7 EcoRI Cloning Site EcoRI PmeI PstI SpeI NotI pCR4-TOPO T7.

PCR Product Orientation Test T7 EcoRI Cloning Site EcoRI PmeI PstI SpeI NotI pCR4-TOPO T7.
IGEM2009 SHORT COURSE Lesson1 Introduction to iGEM and Synthetic Biology.

IGEM2009 SHORT COURSE Lesson1 Introduction to iGEM and Synthetic Biology.
“Fuel it up” Group 24 Sanju Timilsina Parul Sirohi 1.

“Fuel it up” Group 24 Sanju Timilsina Parul Sirohi 1.
Estimating Products.

Estimating Products.
Tina Khoury Jeremy Gerbig Derek Blanchard Kerwin Dunham.

Tina Khoury Jeremy Gerbig Derek Blanchard Kerwin Dunham.

Similar presentations

Ads by Google