1. In vivo homing of intravenously injected CSNRDARRC peptide to bladder tumor in rats.
In vivo homing of intravenously injected CSNRDARRC peptide to bladder tumor in rats. The fluorescein-labeled peptide (500 μL of a 1 mmol/L solution) was injected into the tail vein of bladder tumor–bearing (A-D and G-H) or normal (E and F) rats, or mice bearing a melanoma xenograft (I and J), and allowed to circulate for 2 h. The CSNRDARRC peptide localization (green) in a bladder tumor (B). Costaining for cytokeratin 18 (red) and the merge (C and D). The localization of the CSNRDARRC peptide together with cytokeratin 18 is shown for normal bladder (F), or with 4′,6-diamidino-2-phenylindole in lung (H), and B16/F10 mouse melanoma (J). H&E staining (A, E, G, and I). Magnification, ×400 (A-D) and ×200 (E-J). K. Quantification of fluorescence intensity. The average intensities (integrated intensity per unit area) of fluorescence in tumor and normal tissues (n = 3) were calculated. *, P < 0.05, ANOVA test.
Seung-Min Lee et al. Mol Cancer Res 2007;5:11-19
©2007 by American Association for Cancer Research