1. Fig. 6. Prolonged miR overexpression in the adult heart reduces fibrotic scar size but compromises cardiac function after MI.
Prolonged miR overexpression in the adult heart reduces fibrotic scar size but compromises cardiac function after MI. (A) Study design of miR overexpression by tamoxifen intraperitoneal injection after MI by ligation of the left anterior descending (LAD) coronary artery. (B) Masson’s trichrome–stained heart sections from the site of ligation toward the apex of control and Myh6mercremer:R26R-miR Tg/+ mice at 21 days after MI. Serial sections were cut at 500-μm intervals from the site of the ligature toward the apex. One representative Myh6mercremer and two Myh6mercremer:R26R-miR Tg/+ hearts are shown (n = 6 per group). (C) Quantification of the fibrotic regions in heart sections in (B). (D) Immunostaining and quantification of PH3+/α-actinin+ cells in Myh6mercremer and Myh6mercremer:R26R-miR Tg/+ hearts at 21 days after MI. (E) Cardiac function in mice subjected to LAD ligation, evaluated by echocardiography (n = 7 per group). EF, ejection fraction; FS, fractional shortening; EDV, end-diastolic volume; ESV, end-systolic volume. Data are means ± SEM (n = 6 to 7). (C and D) P values determined with Student’s t test. (E) *P < 0.05 versus sham, by one-way analysis of variance (ANOVA).
Ying Tian et al., Sci Transl Med 2015;7:279ra38
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