1. Specificity of the MeVA RT-qPCR assay, using an Applied Biosystems 7500 platform.
Specificity of the MeVA RT-qPCR assay, using an Applied Biosystems 7500 platform. Synthetic RNA from the six active wild-type measles virus genotypes (B3, D4, D8, D9, G3, and H1) was tested. Panel A shows detection of 107 copies of RNA/reaction in the MeV qPCR assay, and panel B shows the lack of amplification of 107 copies of RNA/reaction in the MeVA RT-qPCR assay. The QuantiTect Probe RT-PCR kit was used for these reactions.
Felicia Roy et al. J. Clin. Microbiol. 2017; doi: /JCM