1. Adipocytes increase melanoma cell lipid content via adipocyte/melanoma lipid transfer.
Adipocytes increase melanoma cell lipid content via adipocyte/melanoma lipid transfer. A, LipidTOX staining on ZMEL1-GFP, SKMel28-GFP, and A375-GFP cells that were cocultured with 3T3L1 adipocytes for 7 days and then FACS isolated. LipidTOX staining intensity was quantified and calculated as fold change of LipidTOX staining in cocultured cells compared with monoculture controls. Each data point represents an average of >10 fields/condition per independent experiment. Mean is shown. Two-tailed unpaired t test; n = 3 independent experiments. B, Transmission electron microscopy of A375-GFP cells FACS isolated after coculture with 3T3L1 adipocytes for 7 days compared with monoculture controls. Error bars, SD. Two-way unpaired t test with Welch correction, n = 10 cells/condition. Scale bar, 2 μm. Representative images are shown. C, Transfer of BODIPY fluorescent fatty acid from adipocytes to melanoma cells. 3T3L1 adipocytes were grown alone and lipid droplets were labeled with BODIPY. After washing away extracellular BODIPY, ZMEL1-GFP, SKMel28-GFP, or A375-GFP cells were plated on top and cocultured with BODIPY-labeled adipocytes for 24 hours and then fixed and imaged. Representative images shown; n = 3 independent experiments. Scale bars, 10 μm. D, 3T3L1 adipocytes were grown alone on the top portion of a Transwell and lipid droplets (LD) were labeled with BODIPY. After washing away extracellular BODIPY, Transwell inserts containing adipocytes were transferred to a new well containing ZMEL1-GFP, SKMel28-GFP, or A375-GFP cells. E, ZMEL1-GFP, SKMel28-GFP, or A375-GFP cells were cocultured for 24 hours and then fixed and imaged. Representative images shown; n = 3 independent experiments. Scale bars, 10 μm.
Maomao Zhang et al. Cancer Discov 2018;8:
©2018 by American Association for Cancer Research