1. Time-dependent changes in caspase-3 during reperfusion after 2 hr middle cerebral artery occlusion.
Time-dependent changes in caspase-3 during reperfusion after 2 hr middle cerebral artery occlusion. Brain lysates (10 μg/lane) were subjected to SDS-PAGE and immunoblot analysis using either caspase-3p32 (A) or caspase-3p20 (B) antiserum as described in Figure 2. Caspase-3p32 band was present in normal brain and did not change over time when measured in four different experiments (see Materials and Methods). Cleavage product (p20) increased 1–6 hr after reperfusion in the left (L, ischemic) compared with right (R, contralateral) and normal brain (N). A faint p20 band is also present at 12 hr. α-tubulin was used as an internal control (bottom). The experiment was repeated four times; a representative experiment is shown.
Shobu Namura et al. J. Neurosci. 1998;18:
©1998 by Society for Neuroscience