1. The p97/VCP ATPase is critical in muscle atrophy and the accelerated degradation of muscle proteins
The content of p97 and two of its cofactors are induced together with proteasomal subunits and the shuttling factor mR23A in Gastrocnemius 10 days after denervation. (A, B) Levels of p97 and its cofactors Ufd1, p47 and Ufd2 (A) and of the proteasomal subunit of the 19S regulatory particle Rpt1, the 20S catalytic core and the shuttling factor mR23A (B) were measured by immunoblotting on extracts from mouse Gastrocnemius at different times after cutting the sciatic nerve. Equal amounts of protein were loaded. Densitometry data and statistical analysis of protein levels are plotted. Error bars indicate s.e.m. (A) *P<0.05, n=6. (B) **P<0.01 and ***P<0.005, n=7. (C) Gastrocnemius weight was decreased 10 days after denervation. *P<0.001, n=4.
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EMBO J, Volume: 31, Issue: 15, Pages: , First published: 06 July 2012, DOI: ( /emboj )