1. The Arabidopsis CERK1‐associated kinase PBL27 connects chitin perception to MAPK activation
Schematic diagram of MAPKKK5 constructs. N: N‐terminal domain, KD: kinase domain, C: C‐terminal domain. MAPKKK5‐C interacts with PBL27 in yeast two‐hybrid experiments. The growth of yeast colonies on plates (‐ULWH) lacking uracil (U), leucine (L), tryptophan (W), and histidine (H) with 2 mM 3‐aminotriazole (3‐AT) indicates a positive interaction. Chitin‐induced MAPK activation was analyzed by immunoblots with α‐pMAPK. Complementation of chitin‐induced MAPK activation in mapkkk5 mutants by expression of MAPKKK5‐FLAG. MAPK activation was analyzed by immunoblots with α‐pMAPK. Flg22‐induced MAPK activation was analyzed by immunoblots with α‐pMAPK. Data information: Experiments (B–E) were performed three times with similar results.
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EMBO J, Volume: 35, Issue: 22, Pages: , First published: 27 September 2016, DOI: ( /embj )