1. The Arabidopsis CERK1‐associated kinase PBL27 connects chitin perception to MAPK activation
The neighbor‐joining phylogenetic tree was created using ClustalW on the DNA Data Bank of Japan ( The full‐length protein sequence for each MAPKKK was used for the alignment. The phylogenetic tree was generated from the modified alignment with Treeview X software. The growth of yeast colonies on plates (‐ULWH) lacking uracil (U), leucine (L), tryptophan (W), and histidine (H) with 5 mM 3‐aminotriazole (3‐AT) indicates a positive interaction. The 3‐AT is a competitive inhibitor of the HIS3 gene product (histidine synthase), which is the reporter gene for the interaction in the yeast two‐hybrid assay. FL: full length, N: N‐terminal domain, KD: kinase domain, C: C‐terminal domain.
IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER.
EMBO J, Volume: 35, Issue: 22, Pages: , First published: 27 September 2016, DOI: ( /embj )