1. Supplementary Fig. 1 bp E F G H I Hybrid 1 SP2/0 MW P3X (-) 850 650
500
850 E F G
400 H I MW
SP2/0
P3X
Hybrid 1
(-)
Supplementary Fig. 1. PCR amplification of antibody heavy chain variable region from hybridoma-1 cell line using alternate primers. Following total RNA isolation and cDNA synthesis, heavy chain VR region from hybridoma-1 cell line (Hybrid-1) was amplified by PCR using 5 alternate PCR primer sets (E-I) and each product resolved by electrophoresis on 1.2% agarose gel. SP2/0 and P3X = parental myeloma cell lines. MW = molecular weight. bp = base pairs. (-) no template control.