1. CNOT6L couples the selective degradation of maternal transcripts to meiotic cell cycle progression in mouse oocyte
Live‐cell imaging results showing in vitro meiotic division of WT and Cnot6l−/− oocytes. Abbreviations: PM, prometaphase; MI, metaphase I; AI, anaphase I; TI, anaphase I‐metaphase II transition; MII, metaphase II; PB1: polar body‐1. Hours after released from GV arrest are indicated. Scale bar, 20 μm. Data represent the mean and standard deviations of mCherry‐securin fluorescence intensity in WT and Cnot6l−/− oocytes at each time point. Values from individual oocytes are normalized relative to that at 0 h after released from GV arrest. The numbers of analyzed oocytes are indicated (n). SMC3 immunofluorescence showing cohesin on chromosomes of WT and Cnot6l−/− oocytes at 16 h after culture. Centromeres and DNA were labeled by CREST and DAPI, respectively. Scale bar, 5 μm. BUB3 immunofluorescence on chromosome spreads made from WT and Cnot6l−/− oocytes at 9.5 h after culture. More than 8 oocytes were observed for each genotype with similar results. Scale bar, 5 μm. PB1 emission rates in WT and Cnot6l−/− oocytes cultured with or without MPS1 inhibitor reversin (5 μm). Reversin is added at 9 h after culture. Error bars, SEM. The numbers of analyzed oocytes are indicated (n).
IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER.
EMBO J, Volume: 37, Issue: 24, First published: 26 November 2018, DOI: ( /embj )