1. A Comparison of Leaf and Petal Senescence in Wallflower Reveals Common and Distinct Patterns of Gene Expression and Physiology
by Anna Marie Price, Danilo F. Aros Orellana, Faezah Mohd Salleh, Ryan Stevens, Rosemary Acock, Vicky Buchanan-Wollaston, Anthony D. Stead, and Hilary J. Rogers
Plantphysiol
Volume 147(4):
August 4, 2008
©2008 by American Society of Plant Biologists

2. Stages of wallflower flower development.
Stages of wallflower flower development. Stage −2 and stage −1, Two sequential buds below the lowest unopened bud on the raceme. Sepals completely cover petals. Stage 0, Lowest unopened bud on raceme. Petals are dark purple in color, tightly curled within sepals. Stage 1, Flower fully opened. Petals are pale purple, with sepals folded back midway along their length. Stigma is yellow and fuzzy in appearance, four of six anthers are visible, all undehisced, positioned close to the stigma with the tips curled over the stigma. Stage 2, As stage 1, but petals are darker in color. All six anthers are visible, two newly emerged anthers are dehisced and curled back from the stigma. Stage 3, The flower is not as tightly held together as previously. Petals are wilting slightly and darker again in color. Fuzz on stigma is not as fine as previously. All six anthers are dehisced and curled back from the stigma. Stage 4, The flower is loosely held together. Petals are limp and curled over at the tips. Flower appearance has deteriorated. Stage 5, As stage 4, but more extreme. Petals are wilted, stigma is discolored with dark purple areas. Stage 6, Sepals, petals, and stamens are beginning to abscise. Remaining petals look withered and dry. Stage 7, All sepals, petals, and stamens are abscised; only the stigma remains. Bar = 10 mm.
Price A et al. Plantphysiol 2008;147:
©2008 by American Society of Plant Biologists

3. Stages of wallflower leaf development.
Stages of wallflower leaf development. Stage 1, Very young leaves, less than 50% expanded. Stage 2, Very young leaves, 50% to 75% expanded. Stage 3, Young leaves, 75% to 100% expanded. Stage 4, Mature green leaves. Stage 5, Older mature leaves, green with signs of yellowing on the tip. Stage 6, Old leaves, up to 50% of leaf area yellow. Stage 7, Very old leaves, mostly or all yellow. Below each image is the total chlorophyll for that leaf stage expressed as a percentage of maximum.
Price A et al. Plantphysiol 2008;147:
©2008 by American Society of Plant Biologists

4. Fresh weight (FW), dry weight (DW), and ratio of dry weight to fresh weight during petal (A) and leaf (B) development and senescence.
Fresh weight (FW), dry weight (DW), and ratio of dry weight to fresh weight during petal (A) and leaf (B) development and senescence. Dry weight was determined by drying 20 to 100 petals or leaves at 60°C for 5 d. Error bars represent ± se (n = 3).
Price A et al. Plantphysiol 2008;147:
©2008 by American Society of Plant Biologists

5. Total protein content of petals (A) and leaves (B).
Total protein content of petals (A) and leaves (B). Error bars represent ± se (n = 15).
Price A et al. Plantphysiol 2008;147:
©2008 by American Society of Plant Biologists

6. Putative functional classes of wallflower genes from different microarray expression classes.
Putative functional classes of wallflower genes from different microarray expression classes. Comparison of putative functional classes of genes represented in two expression categories from the microarray analysis. A, Up-regulated in senescent petal but either stably expressed or down-regulated in senescent leaf. B, Up-regulated in both senescent petal and senescent leaf. Functional classes were derived from Gene Ontogeny annotations and from putative functions based on sequence homology.
Price A et al. Plantphysiol 2008;147:
©2008 by American Society of Plant Biologists

7. RT-PCR of selected genes from the SSH libraries.
RT-PCR of selected genes from the SSH libraries. Semiquantitative RT-PCR over petal (A) and leaf (B), young (Y) and old (O) stages as defined in the text, expressed as percentage of maximum value ± se (n ≥ 3) for SAG12, WLS63, and WC11. Note that data for WLS63 and WC11 expression levels for stage 7 leaves were not determined.
Price A et al. Plantphysiol 2008;147:
©2008 by American Society of Plant Biologists

8. Northern analysis of 10 Arabidopsis genes represented on the microarrays.
Northern analysis of 10 Arabidopsis genes represented on the microarrays. MG represents mature Arabidopsis green leaf with maximum chlorophyll levels (100%); S1 and S2 are stages of Arabidopsis leaf senescence with 98% and 60% chlorophyll levels, respectively (Buchanan-Wollaston and Ainsworth, 1997).
Price A et al. Plantphysiol 2008;147:
©2008 by American Society of Plant Biologists