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BME treatment increases granzyme B expression in NK 3.3 cells.
BME treatment increases granzyme B expression in NK 3.3 cells. A, Western blot analysis of granzyme B expression in control or BME-treated NK3.3 cells when cocultured with Cal27 and JHU-29 cells. B, Phospho-STAT3/STAT3 expression in control or BME-treated NK3.3 cells cocultured with Cal27 was examined by Western blot using specific antibody. Similarly, lysates from NK cell were untreated or BME-treated were analyzed for phospho-STAT3/STAT3 expression. Blots were reprobed with an antibody to actin for comparison of protein loading in each lane. Densitometric analyses of was performed using ImageJ software. Data are represented as mean ± SD. *, P < 0.05; **, P < 0.01. Sourav Bhattacharya et al. Cancer Prev Res 2017;10: ©2017 by American Association for Cancer Research
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