1. A549 cells internalize NHF-ATP and NHF-ATP colocalizes with HMWFD
A549 cells internalize NHF-ATP and NHF-ATP colocalizes with HMWFD. A, A549 cells were incubated with both 10 μmol/L NHF-ATP (green) and HMWFD (red) for 30 minutes and then examined with fluorescence microscopy and quantified by ImageJ.
A549 cells internalize NHF-ATP and NHF-ATP colocalizes with HMWFD. A, A549 cells were incubated with both 10 μmol/L NHF-ATP (green) and HMWFD (red) for 30 minutes and then examined with fluorescence microscopy and quantified by ImageJ. Inlets show colocalization of the two molecules. Contour of each cell was drawn based on photos of the same but overexposed cells. B, intracellular ATP levels in A549 cells treated with or without extracellular ATP or IPA-3, a macropinocytosis inhibitor. ***, P <
Yanrong Qian et al. Mol Cancer Res 2016;14:
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