1. Fig. 1 Bcl11b removal in Treg cells results in early death of mice and increased CD4+ and CD8+ T cell activation.
Bcl11b removal in Treg cells results in early death of mice and increased CD4+ and CD8+ T cell activation. (A) Kaplan-Meier survival curves of mice with the following genotypes: Bcl11bF/F Foxp3YFP-Cre+ (Bcl11b/Treg KO) and Bcl11bF/WT Foxp3YFP-Cre+ (HT) male and Bcl11bF/F Foxp3YFP-Cre+/+ (Bcl11b/Treg KO) and Bcl11bF/WT Foxp3YFP-Cre+/+ (HT) female. (B) Matched weights of Bcl11b/Treg KO and HT male littermates and female littermates at 3 to 4 weeks of age. (C) Peripheral lymph nodes (peLNs) and spleens of 3- to 4-week-old Bcl11b/Treg KO and HT mice. Hematoxylin and eosin (H&E) staining showing the architecture of peLN and spleen. (D) H&E staining of skin and lung samples of 3- to 4-week-old Bcl11b/Treg KO and HT mice. (E) Average frequencies of CD8+ T cells in peLNs and spleens of Bcl11b/Treg KO and HT mice. (F) Representative CD44 histograms in CD4+ and CD8+ T cells from peLNs and spleens of Bcl11b/Treg KO and HT mice. (G) Average frequencies of CD44+ CD4+ and CD44+ CD8+ T cells in peLNs and spleens of Bcl11b/Treg KO and HT mice. (H) Representative flow plots of Treg cells in Bcl11b/Treg KO and HT mice isolated from peLNs and spleens. (I) Average frequencies of Treg cells in Bcl11b/Treg KO and HT mice (n = 7). Analysis was conducted on Bcl11b/Treg KO and HT control mice. P values determined by Student’s t test. **P < 0.01, ***P < 0.001, ****P < Photo credit: Mohammad Uddin, University of Florida.
Theodore T. Drashansky et al. Sci Adv 2019;5:eaaw0480
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