1. mTORC1 is required to prevent cellular senescence.
mTORC1 is required to prevent cellular senescence. A and B, syngeneic mice were injected with Eμ-Myc lymphoma cells (tumor #299), treated once daily with everolimus (E) after the development of overt malignancy, and sacrificed after 0, 2, 4, 7, and 11 days of therapy (n = 4 mice per group). A, sections from lymph nodes of untreated mice and mice sacrificed 2, 4, 7, and 11 days after chronic daily dosing with everolimus were stained with hematoxylin and eosin (H&E), SA-β-gal, anti-Gr1, and anti-F4/80. B, expression of phospho- (P-S15 p53) and total p53, p21, p19ARF, phospho- (P-p38MAPK) and total p38 MAPK, phospho- (P-RPS6), and total RPS6 and H3K9me3 in tumor lysates by Western blotting. Actin was used as a loading control. Full-length blots are presented in Supplementary Fig. S12. C, Four-week-old Eμ-Myc mice were treated with daily placebo (n = 4) or everolimus (n = 4) and sacrificed after 4 days. Splenic sections were stained with H&E (top), or SA-β-gal (bottom).
Meaghan Wall et al. Cancer Discovery 2013;3:82-95
©2013 by American Association for Cancer Research