1. ROS generation at reoxygenation is attributable to NADPH oxidase and is calcium dependent.
ROS generation at reoxygenation is attributable to NADPH oxidase and is calcium dependent. The rate of ROS generation at reoxygenation after OGD was much reduced in neurons from gp91phox−/− knock-out mice (A) or in Ca2+-free saline (0.5 mm EGTA) (B). C and D show that OGD caused a rise in [Ca2+]c in neurons and [Ca2+]c oscillations in astrocytes during OGD. The responses were dependent on external calcium (D). The traces in A and B represent the mean value of the signal measured from all neurons in one of three to seven experiments, whereas C and D show signals from single representative cells.
Andrey Y. Abramov et al. J. Neurosci. 2007;27:
©2007 by Society for Neuroscience