1. DCH-mediated cytotoxicity of isolated CD4+ and CD8+ T cells.
DCH-mediated cytotoxicity of isolated CD4+ and CD8+ T cells. A two-color FACS®-based assay was performed. Kato III target cells were prelabeled with PKH26 and mixed with isolated human CD4+ or CD8+ T cells at an E/T ratio of 10:1. We incubated 10 µg/ml DCH with the cells for 16 h at 37˚C and 5% CO2. A control reaction without fusion protein was included. PI was added to discriminate between live and dead target cells. Error bars indicate standard deviations from duplicate determinations.
Juergen M. Schanzer et al. Cancer Immun 2006;6:4
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