1. Expression of FGF2, IGF-1R, and IR in melanoma cells compared with normal melanocytes.
Expression of FGF2, IGF-1R, and IR in melanoma cells compared with normal melanocytes. A, FGF2 levels in normal and malignant melanocytes as measured by ELISA. Normal melanocytes (NM) were incubated for 16 h in basal medium devoid of TICVA (−) or grown continuously in TICVA (+). Primary melanoma cells WW165 (WW) were grown continuously in basal medium plus IBMX, whereas the metastatic melanoma strains 501 mel (501) YUSIT1 (SIT), YUSAC2 (SAC), YUBSM14 (BSM), and YUDAN3 (DAN) were grown in basal medium. B, expression of IGF-1R and IR in proliferating normal melanocytes (NM) and melanoma cells YUGEN8 (GEN), 501 mel (501), YUSIT1 (SIT), YUSAC2 (SAC), YUBSM14 (BSM), WM9, and YUFIS15 (FIS). The figure shows Western blots of cell extracts with antibodies against IGF-1Rβ and IRβ, using anti-actin as a control for protein loading in each well. Protein markers are indicated by bars here and in all other blots in kDa.
Maria von Willebrand et al. Cancer Res 2003;63:
©2003 by American Association for Cancer Research