1. Fig. 3 Transcriptional changes in dKO mice.
Transcriptional changes in dKO mice. (A) Volcano plot showing the significance of differentially expressed genes in iBAT of dKO versus control mice in RNA-seq Young. The total number of up-regulated (red) and down-regulated (black) genes is shown above the plot. Red dots are considered significant with an adjusted P < 0.1. (B) Network representation of functional enriched biological processes and pathways. Up-regulated genes in dKO were found enriched in several pathways [Enrichr, Fisher Test; false discovery rate (FDR) < 0.05, table S2] and grouped on the basis of the pathways they belong to. Pathways and processes were further grouped into four higher hierarchy super pathways after visually inspecting the clusters. The number of genes contained in each super pathway is shown in parentheses next to the pathway name. (C) Heatmap of normalized (Z score on samples) levels of expression of RT-qPCR–validated genes (in fig. S3) grouped by the three super clusters of interest in (B).
Simona Pedrotti et al. Sci Adv 2019;5:eaav1472
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