1. Effects of WDL on in vitro invasion and soft agar colony formation by LNCaP cells.
Effects of WDL on in vitro invasion and soft agar colony formation by LNCaP cells. A, effect of WDL on invasive capability of LNCaP cells was assayed using Matrigel-coated modified Boyden Transwell chambers as described in the Materials and Methods section. After incubation, cells were fixed and stained with crystal violet. Pictures were taken with a Leica microscope at ×200. B, quantitative measurements of the number of invaded cells with or without drug treatment. Results represent mean values of individual data point ± SD (n = 3). ***, P ≤ ; ****, P ≤ C, effects of WDL and ibuprofen on soft agar colony formation by LNCaP cells are shown. Cells were plated on soft agar in RPMI medium as described in the Materials and Methods section. After incubation for 3 weeks, cells were stained with crystal violet, and growing colonies were counted under microscope at ×150. D, growing colonies were counted, and the results are shown quantitatively as mean values of each data point ± SD (n = 3). ***, P ≤ ; ****, P ≤
Sivalokanathan Sarveswaran et al. Mol Cancer Ther 2016;15:
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