1. WP5: Organic farming Paul Mäder, Sarah Symanczik; FiBL (12)
Andreas de Neergaard, UCPH (8)
Ellen Kandeler, UHOH (1)
Zlata Holeckova, Martin Kulhanek, Pavel Tlustos, Jiri Balik, CULS (3)
Barbara Biro, CUB (5)
A. Piccolo, UNINA (7)
Shekhar Sharma, AFBI (9)
John O’Sullivan, BIOAT (10)
Jörg Geistlinger, AUAS (11)

2. Task 5 - Partner: CULS (03) Hypothesis Aim
BEs will work in the soil with similar properties where the microorganisms were isolated, it means low P and high pH value
By P defficiency – BEs will release P from rock phosphate
Aim
To evaluate the BEs influence on plant height and yields
Measurement of phosphatase activity
P fractionation

3. Task 5 - Partner: CULS (03) Materials and methods
Pot experiment with spring wheat (var. Granny)
Soil
Cítov (50o23‘01,26’’ N; 14o25’11,56’’E)
P content 14.4 mg/kg (Mehlich 3).
The „very low“ P cathegory for Mehlich 3 is under 50 mg P/kg
pHCaCl2 value: 7.7
Chernozem, loamy texture
Mixed with sand 2:1 (soil:sand) – 5 kg of dry substrate/pot
6 replications per treatment

4. Task 5 - Partner: CULS (03) Materials and methods Wheat
Sown at 12th April
25 seeds/pot
21th April selected on 20 plants/pot
Watering on 50 % WHC

5. Task 5 - Partner: CULS (03) Fertilizing Bioeffectors
Nitrogen – 1.5 g N/pot (NH4NO3; mixed with substrate)
Phosphorus – 0.25 g/pot (rock P or TSP; mixed with substrate)
Bioeffectors
Proradix (PDX)
2x109 cfu per pot
CombiFect B without microelements (CFB)
Trichoderma harzianum, OMG16 – 2.5x107 cfu per pot
Bacillus amyloliquefaciens, FZB42 – 2.0x109 cfu per pot
Both BEs were applied locally to the roots in 5 holes (10 cm depth) in the form of 5 ml of water solution per hole.

6. Depo for BEs application
Task 5 - Partner: CULS (03)
Materials and methods
Wheat
Sown at 12th April
25 seeds/pot – 21th April selected on 20 seeds/pot
Watering on 50 % WHC
Randomization – once per week
Depo for BEs application
(10 cm depth)

7. Design of the experiment
Task 5 - Partner: CULS (03)
Design of the experiment
Nr.
Bioeffectors
P fertilizing 1
BE0 P0 2
Proradix 3
CombiFect B 4 RP 5 6 7
TSP 8 9
PDX P0
PDX RP
PDX TSP

8. Results – plant height (in cm) – 12.5.
Task 5 - Partner: CULS (03)
Results – plant height (in cm) – 12.5.
Tukey test p≤0.05
Different letters presents significant differences

9. Results – plant height (in cm) – 12.5.
Task 5 - Partner: CULS (03)
Results – plant height (in cm) – 12.5.
Tukey test p≤0.05
Different letters presents significant differences

10. Results – plant height (in cm) – 14.6.
Task 5 - Partner: CULS (03)
Results – plant height (in cm) – 14.6.
Tukey test p≤0.05
Different letters presents significant differences

11. Task 5 - Partner: CULS (03) First basic results Running analyses
Positive influence of Proradix without P fertilizing on plant height in early vegetation stages
Running analyses
Acid and alkaline phosphatase activity in soil
Next analyses
Phosphorus fractionation focused on
Water extractable phosphorus
Exchangeable phosphorus
Microbial biomass P