1. Distribution of γ-H2AX foci on metaphase spreads from BLM-proficient and BLM-deficient cells.
Distribution of γ-H2AX foci on metaphase spreads from BLM-proficient and BLM-deficient cells. Representative metaphase spreads were obtained from PSNF5 and PSNG13 cells. Red, telomeres labeled by fluorescence in situ hybridization. Green, γ-H2AX foci. Blue, chromosomes stained with DAPI. The colocalization of the red and green staining indicates a telomeric position of the γ-H2AX foci, which are referred to as chromosome-ends foci (A). The green signals localized within the chromosomes are referred to as intrachromosome foci (B). C. Quantification of the γ-H2AX foci per metaphase and their chromosomal distribution in BLM-proficient (PSNF5) and BLM-deficient (PSNG13) cells. The majority of the γ-H2AX foci were located at the chromosome ends in both cell lines. The percentages of chromosome-end foci were the same in both cell lines. Bars, SD of three independent experiments. Forty and thirty four metaphases were analyzed for PSNF5 and PSNG13, respectively.
V. Ashutosh Rao et al. Mol Cancer Res 2007;5:
©2007 by American Association for Cancer Research