1. Enhancement of MYC-related HAT complex recruitment to the target genes and histone H4 acetylation by MAPJD. A, interaction of MAPJD with HAT complex containing MYC. Immunoprecipitation with antibodies to endogenous MYC, TRRAP, or TIP60 followed by immunoblo...
Enhancement of MYC-related HAT complex recruitment to the target genes and histone H4 acetylation by MAPJD. A, interaction of MAPJD with HAT complex containing MYC. Immunoprecipitation with antibodies to endogenous MYC, TRRAP, or TIP60 followed by immunoblotting with anti-FLAG antibody (M2), using extracts of LC319 cells transfected with FLAG-MAPJD–expressing or mock vectors. B, association of acetylated histone H4 (AcH4) and endogenous TRRAP with DNA fragments of 5′-flanking region containing E-boxes of the four MAPJD target genes, SBNO1, TGFBRAP1, RIOK1, and RASGEF1A, detected by ChIP assay. DNAs from LC319 cells transfected with MAPJD-expressing or mock vectors were immunoprecipitated with indicated antibodies and served for PCR. CHRNB (cholinergic receptor, nicotinic, β1, and muscle; see ref. 41) was used as a quantitative control.
Chie Suzuki et al. Mol Cancer Ther 2007;6:
©2007 by American Association for Cancer Research