1. Effects of the genotypes of Rapgef2 and Rapgef6 on the apical surface structures.
Effects of the genotypes of Rapgef2 and Rapgef6 on the apical surface structures. A, B, Immunohistological detection of proteins constituting the AJs. A, B, Coronal sections of the brains of Rapgef2-f/f (2-f/f), Rapgef2-cKO (2-cKO), Rapgef6-KO (6-KO), and Rapgef2/6-dKO (2/6-dKO) embryos at E13.5 (A) and E15.5 (B) were subjected to immunostaining for β-catenin (β-cat.), N-cadherin (N-cad.), E-cadherin (E-cad.), afadin, and ZO-1, as indicated. The leftmost panels are phase contrast images with a lower magnification. The images are representative of four biological replicates of each group. Scale bars: leftmost panels, 100 µm; other panels, 10 µm.
Kazuhiro Maeta et al. eneuro 2016;3:ENEURO
©2016 by Society for Neuroscience