1. GFC neurons support GF circuit architectural development.
GFC neurons support GF circuit architectural development. A, The GFI labeled by iontophoretically injected TRITC (magenta) reveals the soma (arrow) and dendritic branches (arrowheads) in the brain (top), and descending axon in thoracic ganglion (bottom). Split Gal4 (spGal4) 10B11-AD ∩ 14A06-DBD drives UAS-mCD8::GFP (green) in GFC1 (bottom, arrow) and PSI (bottom, arrowhead). B, Iontophoretic NB injection into the GFI (yellow) in the UAS-hid/+ control reveals the GFI (arrows) interconnected by the GCI (arrowheads) in the brain (top) and normal dye coupling in the thoracic ganglion (bottom). C, Driving UAS-hid with spGal4 10B11-AD ∩ 14A06-DBD results in the loss of GFC1 with occasional PSI survival (arrowhead). When GFC1 is ablated, the GCI labeling is often lost (top), one of the GFI axons is typically absent, and the remaining GFI axon always extends a compensatory contralateral axon projection (arrow). All NB injections were performed on males. D, Schematic representations of GF circuit outcomes with UAS-hid/+ controls and spGal4 10B11-AD ∩ 14A06-DBD-driven UAS-hid cell ablation. Not pictured are instances where neither GFC1 nor PSI are ablated, and instances where both GFIs are absent. E, Frequency of each GF circuit outcome with the targeted spGal4 10B11-AD ∩ 14A06-DBD-driven UAS-hid cell ablation. The pie chart color is coded to dots at the bottom of schematics in D. The sample size for UAS-hid/+ genetic controls is 21 animals, and for the spGal4 cell ablation it is 20 animals.
Tyler Kennedy, and Kendal Broadie eNeuro 2018;5:ENEURO
©2018 by Society for Neuroscience