1. SPDO isoforms are expressed differentially during development and in adult tissues.
SPDO isoforms are expressed differentially during development and in adult tissues. (A) A data analysis program (GadFly) ( which searches expressed sequence tags (ESTs) from the Drosophila genome project predicts two alternative promoters (black exons) used to generate SPDO isoforms. CT41421 and CT3919 are mRNA transcripts predicted to generate 366 AA (43 kD) and 402 AA (45 kD) protein products, respectively. Arrow represents translational start sites that contribute 2 or 36 AA for CT4121 and CT 3919, respectively. (B) Western Blot prepared from heterozygous (spdo/+) or homozygous (spdo/spdo) spdo embryos probed with mouse anti-SPDO antibody (α-SPDO) (left) or anti–human erythrocyte Tmod antibody (α-Tmod) (right). (C) Western blot prepared from larvae or dissected adult flies probed with anti-SPDO antibodies.
Michelle Mardahl-Dumesnil, and Velia M. Fowler J Cell Biol 2001;155:
© 2001 Rockefeller University Press