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A, scatterplots of qMSP analysis of candidate genes promoters in the prevalence screen cohort, which consisted of 55 HNSCC tumor tissue samples and 37 normal tissue samples obtained from UPPP procedures carried out in noncancer patients. A, scatterplots of qMSP analysis of candidate genes promoters in the prevalence screen cohort, which consisted of 55 HNSCC tumor tissue samples and 37 normal tissue samples obtained from UPPP procedures carried out in noncancer patients. The relative level of methylated DNA for each gene in each sample was determined as a ratio of MSP for the amplified gene to ACTB and then multiplied by 100 [(average value of duplicates of gene of interest÷average value of duplicates of ACTB) × 100] for HOXA9 and NID2. Red line denotes cutoff value. B, ROC curve of HOXA9 (solid line) and NID2 (dashed line) methylation in a HNSCC prevalence cohort (n = 92). R. Guerrero-Preston et al. Cancer Prev Res 2011;4: ©2011 by American Association for Cancer Research
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