1. Inhibition of stress-associated MAPK signaling attenuates SASP expression in CAFs. A, N-CAFs were treated with 100 nmol/L GEM plus DMSO or the above-listed small-molecule inhibitors for 48 hours, and SASP mediator expression was compared with that of untrea...
Inhibition of stress-associated MAPK signaling attenuates SASP expression in CAFs. A, N-CAFs were treated with 100 nmol/L GEM plus DMSO or the above-listed small-molecule inhibitors for 48 hours, and SASP mediator expression was compared with that of untreated cells. Drug concentrations and targets: SB μmol/L, P38 MAPK; SP μmol/L, JNK; BEZ235 1 μmol/L, ATM/ATR/DNA-PK/PI3K/mTOR; AZD nmol/L, Chk1/Chk2, VE821 2 μmol/L ATR; KU μmol/L, ATM. B, cell lysates from N-CAFs treated with 100 nmol/L GEM and DMSO, SB203580, or SP at the indicated concentrations (μmol/L) were blotted for P38 MAPK and JNK activation as well as their downstream targets, HSP27 and cJUN, respectively. Lysates from untreated N-CAFs were used as a control. Densitometry values are displayed below the blots. All values were normalized to GAPDH, and expression is displayed as the ratio of phospho- to total protein. For all experiments, data represent the composite of two separately performed experiments. The blots displayed in B are from one representative experiment.
Paul A. Toste et al. Mol Cancer Res 2016;14:
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