1. The expression of VCX3A and other CT antigens in pediatric HGG
The expression of VCX3A and other CT antigens in pediatric HGG. A, Immunohistochemistry for VCX3A protein in pediatric HGG. The tissue microarrays were stained using SuperPicture 3rd Gen IHC Detection Kit (Thermo Fisher Scientific).
The expression of VCX3A and other CT antigens in pediatric HGG. A, Immunohistochemistry for VCX3A protein in pediatric HGG. The tissue microarrays were stained using SuperPicture 3rd Gen IHC Detection Kit (Thermo Fisher Scientific). Cell nuclei were counterstained with hematoxylin. The genotype of H3F3A was indicated on the top of each panel. K27M, histone H3 lysine27methionine mutation; scale bar, 50 μm. B, The bar plot of VCX3A expression in pediatric HGG stratified by H3F3A status, depicting the percentage of cases with the indicated signal grades. –, negative; +/–, equivocal; +, weak positive; ++, strong positive. C, GSEA of a signature gene set for CT antigens in pediatric HGG with H3F3AK27M mutation versus those with WT H3F3A. The signature gene set consists of the full list of CT antigens curated in CTdatabase ( Publicly available pHGG gene expression profiles (GSE34824, GSE36245, and GSE49822) were processed using RMA (quantile normalization), combined and stratified for H3F3A status [H3F3A K27M (K27M), n = 14; H3F3A WT, n = 51] as per the published sample annotation, and then subjected to the analysis. The normalized enrichment score (NES), the nominal P value (NOM P-val), and the false discovery rate Q value (FDR q val) are shown at the upper right corner of the graph. D, Upregulation of IL13RA2 in pHGG with the H3.3K27M mutation. The gene expression data of IL13RA2 were retrieved from Gene Expression Omnibus (GEO) of NCBI (GSE34824, GSE36245, and GSE49822).
Houliang Deng et al. Mol Cancer Res 2018;16:
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