1. Downregulation of ROCK signaling in striatal tissues from SA/SA mutant mice.
Downregulation of ROCK signaling in striatal tissues from SA/SA mutant mice. A, ROCK activity levels in the striatal tissues of SA/SA (left) and SD/SD (right) mutant mice measured by immunoassays. B, ROCK protein levels in striatal tissues from SA/SA (left) and SD/SD (right) mutant mice measured by Western blotting (upper). ROCK protein level (A.U.) in each tissue was normalized to that of β-tubulin (Tub, lower). C, ROCK kinase activity (mU) per milligram of striatal tissues and normalized to ROCK protein amount (bottom) in SA/SA (left) and SD/SD (right) mutant mice. Scatter diagrams of the two variables are shown in upper panels. D, Phosphorylation of 60-kDa Akt at Ser473 (P-Akt) in the striatal tissues of SA/SA mutant mice as measured by Western blotting. Akt phosphorylation levels were normalized to the level of total Akt. Data are mean ± SD; *p < 0.05, **p < 0.01 by unpaired two-tailed Welch’s t tests.
Goro Kato eNeuro 2019;6:ENEURO
©2019 by Society for Neuroscience